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packard cyclone storage phosphor system  (Packard Instruments)

 
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    Packard Instruments packard cyclone storage phosphor system
    YB-1 and PABP effects on translation of YB-1 mRNA and its mutant forms. (A and C) 0.1 pmol of C+A− wild type, mutated YB-1 mRNAs, and Luciferase mRNA, as internal control, were translated in a rabbit reticulocyte cell-free system in the presence of increasing amounts of YB-1 (3.5 pmol, lanes 2, 7, 12, 17, 22; 7 pmol, lanes 3, 8, 13, 18, 23; 10.5 pmol, lanes 4, 9, 14, 19, 24; 14 pmol, lanes 5, 10, 15, 20, 25) or without it (lanes 1, 6, 11, 16, 21) (A) and in the presence of increasing amounts of PABP (2 pmol, lanes 2, 6, 10, 14, 18; 4 pmol, lanes 3, 7, 11, 15, 19; 6 pmol, lanes 4, 8, 12, 16, 20) or without it (lanes 1, 5, 9, 13, 17) (C). [14C]Tyr-labeled translation products were resolved by SDS-PAGE and visualized by autoradiography. (B and D) The relative amount of radioactivity in the bands of (A and C), respectively, was determined using a <t>Packard</t> Cyclone Storage Phosphor System (Packard Instrument Company, Inc.). The level of translation of each mRNA without addition of YB-1 or PABP was taken to be 100%. Values are the means of at least three independent experiments. Errors are standard deviations. Student test was used to confirm the statistic significance of differences between values for WT and mutant YB-1 mRNAs. ***p < 0.001; **p < 0.01; *p < 0.05.
    Packard Cyclone Storage Phosphor System, supplied by Packard Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/packard cyclone storage phosphor system/product/Packard Instruments
    Average 90 stars, based on 1 article reviews
    packard cyclone storage phosphor system - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "Interplay between Y-box-binding protein 1 (YB-1) and poly(A) binding protein (PABP) in specific regulation of YB-1 mRNA translation"

    Article Title: Interplay between Y-box-binding protein 1 (YB-1) and poly(A) binding protein (PABP) in specific regulation of YB-1 mRNA translation

    Journal: RNA Biology

    doi: 10.4161/rna.8.5.16022

    YB-1 and PABP effects on translation of YB-1 mRNA and its mutant forms. (A and C) 0.1 pmol of C+A− wild type, mutated YB-1 mRNAs, and Luciferase mRNA, as internal control, were translated in a rabbit reticulocyte cell-free system in the presence of increasing amounts of YB-1 (3.5 pmol, lanes 2, 7, 12, 17, 22; 7 pmol, lanes 3, 8, 13, 18, 23; 10.5 pmol, lanes 4, 9, 14, 19, 24; 14 pmol, lanes 5, 10, 15, 20, 25) or without it (lanes 1, 6, 11, 16, 21) (A) and in the presence of increasing amounts of PABP (2 pmol, lanes 2, 6, 10, 14, 18; 4 pmol, lanes 3, 7, 11, 15, 19; 6 pmol, lanes 4, 8, 12, 16, 20) or without it (lanes 1, 5, 9, 13, 17) (C). [14C]Tyr-labeled translation products were resolved by SDS-PAGE and visualized by autoradiography. (B and D) The relative amount of radioactivity in the bands of (A and C), respectively, was determined using a Packard Cyclone Storage Phosphor System (Packard Instrument Company, Inc.). The level of translation of each mRNA without addition of YB-1 or PABP was taken to be 100%. Values are the means of at least three independent experiments. Errors are standard deviations. Student test was used to confirm the statistic significance of differences between values for WT and mutant YB-1 mRNAs. ***p < 0.001; **p < 0.01; *p < 0.05.
    Figure Legend Snippet: YB-1 and PABP effects on translation of YB-1 mRNA and its mutant forms. (A and C) 0.1 pmol of C+A− wild type, mutated YB-1 mRNAs, and Luciferase mRNA, as internal control, were translated in a rabbit reticulocyte cell-free system in the presence of increasing amounts of YB-1 (3.5 pmol, lanes 2, 7, 12, 17, 22; 7 pmol, lanes 3, 8, 13, 18, 23; 10.5 pmol, lanes 4, 9, 14, 19, 24; 14 pmol, lanes 5, 10, 15, 20, 25) or without it (lanes 1, 6, 11, 16, 21) (A) and in the presence of increasing amounts of PABP (2 pmol, lanes 2, 6, 10, 14, 18; 4 pmol, lanes 3, 7, 11, 15, 19; 6 pmol, lanes 4, 8, 12, 16, 20) or without it (lanes 1, 5, 9, 13, 17) (C). [14C]Tyr-labeled translation products were resolved by SDS-PAGE and visualized by autoradiography. (B and D) The relative amount of radioactivity in the bands of (A and C), respectively, was determined using a Packard Cyclone Storage Phosphor System (Packard Instrument Company, Inc.). The level of translation of each mRNA without addition of YB-1 or PABP was taken to be 100%. Values are the means of at least three independent experiments. Errors are standard deviations. Student test was used to confirm the statistic significance of differences between values for WT and mutant YB-1 mRNAs. ***p < 0.001; **p < 0.01; *p < 0.05.

    Techniques Used: Mutagenesis, Luciferase, Labeling, SDS Page, Autoradiography, Radioactivity



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    Packard Instruments packard cyclone storage phosphor system
    YB-1 and PABP effects on translation of YB-1 mRNA and its mutant forms. (A and C) 0.1 pmol of C+A− wild type, mutated YB-1 mRNAs, and Luciferase mRNA, as internal control, were translated in a rabbit reticulocyte cell-free system in the presence of increasing amounts of YB-1 (3.5 pmol, lanes 2, 7, 12, 17, 22; 7 pmol, lanes 3, 8, 13, 18, 23; 10.5 pmol, lanes 4, 9, 14, 19, 24; 14 pmol, lanes 5, 10, 15, 20, 25) or without it (lanes 1, 6, 11, 16, 21) (A) and in the presence of increasing amounts of PABP (2 pmol, lanes 2, 6, 10, 14, 18; 4 pmol, lanes 3, 7, 11, 15, 19; 6 pmol, lanes 4, 8, 12, 16, 20) or without it (lanes 1, 5, 9, 13, 17) (C). [14C]Tyr-labeled translation products were resolved by SDS-PAGE and visualized by autoradiography. (B and D) The relative amount of radioactivity in the bands of (A and C), respectively, was determined using a <t>Packard</t> Cyclone Storage Phosphor System (Packard Instrument Company, Inc.). The level of translation of each mRNA without addition of YB-1 or PABP was taken to be 100%. Values are the means of at least three independent experiments. Errors are standard deviations. Student test was used to confirm the statistic significance of differences between values for WT and mutant YB-1 mRNAs. ***p < 0.001; **p < 0.01; *p < 0.05.
    Packard Cyclone Storage Phosphor System, supplied by Packard Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/packard cyclone storage phosphor system/product/Packard Instruments
    Average 90 stars, based on 1 article reviews
    packard cyclone storage phosphor system - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier
    cyclone® system (storage phosphor system- packard instr)  (Packard Instruments)
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    Packard Instruments cyclone® system (storage phosphor system- packard instr)
    YB-1 and PABP effects on translation of YB-1 mRNA and its mutant forms. (A and C) 0.1 pmol of C+A− wild type, mutated YB-1 mRNAs, and Luciferase mRNA, as internal control, were translated in a rabbit reticulocyte cell-free system in the presence of increasing amounts of YB-1 (3.5 pmol, lanes 2, 7, 12, 17, 22; 7 pmol, lanes 3, 8, 13, 18, 23; 10.5 pmol, lanes 4, 9, 14, 19, 24; 14 pmol, lanes 5, 10, 15, 20, 25) or without it (lanes 1, 6, 11, 16, 21) (A) and in the presence of increasing amounts of PABP (2 pmol, lanes 2, 6, 10, 14, 18; 4 pmol, lanes 3, 7, 11, 15, 19; 6 pmol, lanes 4, 8, 12, 16, 20) or without it (lanes 1, 5, 9, 13, 17) (C). [14C]Tyr-labeled translation products were resolved by SDS-PAGE and visualized by autoradiography. (B and D) The relative amount of radioactivity in the bands of (A and C), respectively, was determined using a <t>Packard</t> Cyclone Storage Phosphor System (Packard Instrument Company, Inc.). The level of translation of each mRNA without addition of YB-1 or PABP was taken to be 100%. Values are the means of at least three independent experiments. Errors are standard deviations. Student test was used to confirm the statistic significance of differences between values for WT and mutant YB-1 mRNAs. ***p < 0.001; **p < 0.01; *p < 0.05.
    Cyclone® System (Storage Phosphor System Packard Instr), supplied by Packard Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cyclone® system (storage phosphor system- packard instr)/product/Packard Instruments
    Average 90 stars, based on 1 article reviews
    cyclone® system (storage phosphor system- packard instr) - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    YB-1 and PABP effects on translation of YB-1 mRNA and its mutant forms. (A and C) 0.1 pmol of C+A− wild type, mutated YB-1 mRNAs, and Luciferase mRNA, as internal control, were translated in a rabbit reticulocyte cell-free system in the presence of increasing amounts of YB-1 (3.5 pmol, lanes 2, 7, 12, 17, 22; 7 pmol, lanes 3, 8, 13, 18, 23; 10.5 pmol, lanes 4, 9, 14, 19, 24; 14 pmol, lanes 5, 10, 15, 20, 25) or without it (lanes 1, 6, 11, 16, 21) (A) and in the presence of increasing amounts of PABP (2 pmol, lanes 2, 6, 10, 14, 18; 4 pmol, lanes 3, 7, 11, 15, 19; 6 pmol, lanes 4, 8, 12, 16, 20) or without it (lanes 1, 5, 9, 13, 17) (C). [14C]Tyr-labeled translation products were resolved by SDS-PAGE and visualized by autoradiography. (B and D) The relative amount of radioactivity in the bands of (A and C), respectively, was determined using a Packard Cyclone Storage Phosphor System (Packard Instrument Company, Inc.). The level of translation of each mRNA without addition of YB-1 or PABP was taken to be 100%. Values are the means of at least three independent experiments. Errors are standard deviations. Student test was used to confirm the statistic significance of differences between values for WT and mutant YB-1 mRNAs. ***p < 0.001; **p < 0.01; *p < 0.05.

    Journal: RNA Biology

    Article Title: Interplay between Y-box-binding protein 1 (YB-1) and poly(A) binding protein (PABP) in specific regulation of YB-1 mRNA translation

    doi: 10.4161/rna.8.5.16022

    Figure Lengend Snippet: YB-1 and PABP effects on translation of YB-1 mRNA and its mutant forms. (A and C) 0.1 pmol of C+A− wild type, mutated YB-1 mRNAs, and Luciferase mRNA, as internal control, were translated in a rabbit reticulocyte cell-free system in the presence of increasing amounts of YB-1 (3.5 pmol, lanes 2, 7, 12, 17, 22; 7 pmol, lanes 3, 8, 13, 18, 23; 10.5 pmol, lanes 4, 9, 14, 19, 24; 14 pmol, lanes 5, 10, 15, 20, 25) or without it (lanes 1, 6, 11, 16, 21) (A) and in the presence of increasing amounts of PABP (2 pmol, lanes 2, 6, 10, 14, 18; 4 pmol, lanes 3, 7, 11, 15, 19; 6 pmol, lanes 4, 8, 12, 16, 20) or without it (lanes 1, 5, 9, 13, 17) (C). [14C]Tyr-labeled translation products were resolved by SDS-PAGE and visualized by autoradiography. (B and D) The relative amount of radioactivity in the bands of (A and C), respectively, was determined using a Packard Cyclone Storage Phosphor System (Packard Instrument Company, Inc.). The level of translation of each mRNA without addition of YB-1 or PABP was taken to be 100%. Values are the means of at least three independent experiments. Errors are standard deviations. Student test was used to confirm the statistic significance of differences between values for WT and mutant YB-1 mRNAs. ***p < 0.001; **p < 0.01; *p < 0.05.

    Article Snippet: The relative amount of radioactivity in the bands was determined using a Packard Cyclone Storage Phosphor System (Packard Instrument Company, Inc.).

    Techniques: Mutagenesis, Luciferase, Labeling, SDS Page, Autoradiography, Radioactivity